Question

You have identified a useful gene in a bacteria. Make a flowchart of the steps that you would follow to transfer this gene to a plant.

Answer 1

After identifying a useful gene in bacteria, following steps should be undertaken

(1) Isolation of useful gene using Restriction Endonucleases

                 ↓  

(2) Transferring the gene to a suitable vector to create a recombinant DNA molecule

                 ↓  

(3) Transfer of these recombinant DNA molecules to the target cells

                ↓  

(4) Screening of cells for transformation

                **↓**  

(5) Selection of transformed cells

             **↓**  

(6) Regeneration of plants from the transformed cells to get transgenic plants.