Question

Observe the given sequence of nitrogenous bases on a DNA fragment and answer the following question –

5´ – CAGAATTCTTA – 3´ 3´ – GTCTTAAGAAT – 5´

(a) Name a restriction enzme which can recognise this DNA sequence.

(b) Write the sequence after digestion.

(c) Why are the ends generated after digestion called sticky ends?

Answer 1

(a) EcoRI

(c) These are named sticky ends, because they form hydrogen bonds with their complementary cut parts.