Question

Students of Class XII visited Microbial Type Culture Collection, Chandigarh and observed microbial cultures of Providencia stuartii, Streptomyces albus and Haemophilus aegyptus. Name the restriction enzymes obtained from them and also specify their restriction sites.

Answer 1

Haemophilus Aegyptus HaeIII

5'G-G-C-C 3’

3'C-C-G-G 5’

Providencia stuartii PstI

5'C-T-G-C-A-G 3’

3'G-A-C-G-T-C 5’

Streptomyces albus SalI

5'G-T-C-G-A-C 3’

3'C-A-G-C-T-G 5'