The technique that will help the authorities to establish the identity of the **** is known as DNA fingerprinting.
Basis of DNA Fingerprinting - DNA fingerprinting is a method for comparing the DNA sequences of any two individuals. 99.9% o{ the base sequences in all human beings are identical. It is the remaining 0.1% that makes every individual unique. In this, certain specific regions called repetitive DNA sequences that are different for every individual are used for comparative study. This repetitive DNA is separated from the bulk DNA as different peaks during density gradient centrifugation in which bulk DNA forms major peak and the other small peaks are referred to as satellite DNA. These sequences show high degree of polymorphism and form the basis of DNA fingerprinting.
Methodology of DNA fingerprinting ---
The DNA fingerprinting technique involves following steps :
(i) Isolation of DNA
(ii) Digestion of DNA by restriction endonuclease
(iii) Separation of DNA fragments by electrophoresis
(iv) Transfer of separated DNA fragments to symthetic membrane
(v) Hybridisation of separated fragments using labelled VNTR probes
(vi) Detection of hybridised DNA fragments by autoradiography After autoradiography, different bands are obtained which are characteristics of an individual. The presence of similarities between the casualties and their relatives determines their relatedness on the basis of which the **** bodies handed over to their respective relative
